6. Simulating light sheet microscopyΒΆ

The described routines form the biobeam-specific, GPU accelerated basis of wave-optical simulation and accurate field implementations, that enable the assembly of piplines to account for rigorous image-formation process of entire microscopes in tissues.

_images/lightsheet.png

A cylindrical light sheet is scanned through a synthetic embryo.

Please see the paper for a description of the steps required to mimic a fluorescent light-sheet microscope. As in practice, its perfomance is determined by the complex interplay of light scattering on the illumination and detection side.